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In this paper, for sensing and monitoring the biochemical analyte dissolved in liquid, antigen-antibody interaction or protein-DNA/RNA binding process, we design a surface plasmon resonance refractive index based biosensor by using a dual core D-shaped six-fold photonic crystal fibre which is embedded with silver nanowires for multi-detection. We numerically analyze both the dispersion relations and the loss spectra for various analytes by finite element method. This optical fibre bio-sensor monitors the changes of the refractive index for different analytes by measuring the spectral shifts of the fibre loss peaks at their resonance wavelengths. With the wavelength interrogation method, we find that the proposed biosensor with two sensing channels exhibits a maximum refractive index sensitivity of 3400 nm/RIU and a resolution of 2.94 × 10 ^-5 RIU for a large sensing range from 1.35 to 1.50, which covers most …