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Fossil fuel combustion in internal combustion engines, especially gasoline engines, leads to urban air pollution and contributes to greenhouse gas emissions and climate change. Ethanol has long been considered a promising option to replace gasoline and reduce the amount of associated pollution. Despite its advantages of improving fuel combustion and reducing pollutants, increasing nitrogen oxide emission is an environmental issue caused by using a high percentage of ethanol in fuel. Another challenge the biofuel industry faces is the crude glycerol produced as a by-product of the biodiesel production process, which requires a high-technology purification line, restricting biodiesel production capacity. Considering both challenges, the present study valorized glycerol into a fuel bio-additive, i.e., a ketal-acetin mixture, and developed gasoline-ethanol fuel formulations containing the synthesized bio-additive …