作者
Hector Carbajal-Contreras, Adrian Rafael Murillo-de-Ozores, Germán Magaña-Avila, Alejandro Marquez-Salinas, Laurent Bourqui, Michelle Tellez-Sutterlin, Jessica P Bahena-Lopez, Eduardo Cortes-Arroyo, Sebastián González Behn-Eschenburg, Alejandro Lopez-Saavedra, Norma Vazquez, David H Ellison, Johannes Loffing, Gerardo Gamba, Maria Castañeda-Bueno
发表日期
2024/2/1
期刊
American Journal of Physiology-Renal Physiology
卷号
326
期号
2
页码范围
F285-F299
出版商
American Physiological Society
简介
Vasopressin regulates water homeostasis via the V2 receptor in the kidney at least in part through protein kinase A (PKA) activation. Vasopressin, through an unknown pathway, upregulates the activity and phosphorylation of Na+-Cl– cotransporter (NCC) and Na+-K+-2Cl– cotransporter 2 (NKCC2) by Ste20-related proline/alanine-rich kinase (SPAK) and oxidative stress-responsive kinase 1 (OSR1), which are regulated by the with-no-lysine kinase (WNK) family. Phosphorylation of WNK4 at PKA consensus motifs may be involved. Inhibitor 1 (I1), a protein phosphatase 1 (PP1) inhibitor, may also play a role. In human embryonic kidney (HEK)-293 cells, we assessed the phosphorylation of WNK4, SPAK, NCC, or NKCC2 in response to forskolin or desmopressin. WNK4 and cotransporter phosphorylation were studied in desmopressin-infused WNK4−/− mice and in tubule suspensions. In HEK-293 cells, only wild-type …
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H Carbajal-Contreras, AR Murillo-de-Ozores… - American Journal of Physiology-Renal Physiology, 2024