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Dyes are one of the pollutants and due to their toxicity, carcinogenesis and irreversible the environment and humans. The efficient removal of various dyes from the waste waters of textile industries is an important and challenging task. In the present study, activated carbon (AC) have been prepared from Delonix regia seeds by chemical activation with H2SO4 and it is attempted to validate the applicability of a new adsorbent for the removal of a cationic dye Methylene blue from the effluent wastewaters. Batch experiments were done to investigate the contact time, effect of pH, effect of adsorbent, initial dye concentration and Langmuir isotherm models were used to describe the interaction between the adsorbate and adsorbent. As the dose increased, removal efficiency increased with statistical significance (P< 0.05). The experimental sorption equilibrium followed by Langmuir (R2= 0.9991) and the RL value was 0.4975 which means Langmuir isotherm is favorable for this treatment. The adsorption behavior of Delonix regia has been investigated to understand the physicochemical behaviour of water like pH, electrical conductivity, salinity, temperature, color, TDS and TSS before and after adsorption. The result found was 5.13±0.0137, 900.00±0.11 µs/cm, 0.40, 27.13±2.82 C, blue, 442.66±0.316 mg/L and 850 mg/L before Adsorption and 6.83±0.27, 362±0.96 µs/cm, 1.87±3.25, 26.63±0.57 C, Colorless, 458.2 mg/L and 540 mg/L after adsorption. This showed that Delonix regia seed are suitable candidates for use as biosorbents in the removal of dyes from aqueous solutions.