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The world is in need of a green, efficient, carbon-neutral energy source to replace fossil fuels. The search for energy alternatives involving locally available and renewable resource is one of the main concerns of governments, scientists, and business people worldwide. Biogas, formed by anaerobic digestion of organic materials makes sustainable, reliable and renewable energy possible. This study involves the construction of a lab scale biogas production plant digester. The production of biogas was carried out by employing the mixed substrates of 13 different fruit and vegetable wastes collected from Arbaminch vegetable market. The materials used as feed were Apple, Mango, Cabbage, Avocado, onion, potato, Tomato, Banana, Lemon, Orange, Carrot, Papaya and cow manure. The total solid, volatile solids, moisture content and ash content of the wastes were examined. The anaerobic digestion of fruit and vegetable wastes mixed with different waste took 35-40 days to produce biogas (for complete digestion). Anaerobic digestion is very sensitive to change in pH and it is important to maintain pH of 6.7-7.4 for healthy system. The process resulted in the production of nutrient rich slurry high C/N ratio. The presented data on the moisture content, total solid, volatile solid and ash content of the wastes shows that tomato had maximum moisture content of 95.02% and lemon had the least with a moisture content of 73.4%. The maximum TS were recorded in Avocado (24.47%). The VS in all wastes used for the study varied from 20%(carrot) to 46.5%(Mango) wastes. The characteristics of these wastes were found to be: TS 14.13% of wet weight, VS 26 …