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Environmental endocrine-disrupting chemicals (EDCs) are a large category of chemicals that can disrupt normal endocrine functions of organisms. They can enter the environment and foodstuffs in different ways and bring great threat to human health. Phenolic environmental estrogens (PEEs) are the typical representatives of EDCs and include bisphenols, alkylphenols, and others such as dibromophenol, dichlorophenol, 4-nitrophenononylphenol, etc. Even trace amounts of PEEs can cause serious damage to the human reproductive, nervous, and immune systems; therefore, it is very important to develop accurate and sensitive methods for their determination in different matrixes. At the present, there are still many challenges in the determination of PEEs in the samples with complex matrixes, and the sample treatment is one of the key issues. This review deals with the recent advances in sample treatment …