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Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape ( Vitis spp.), apple ( Malus spp.), pear ( Pyrus spp.) and persimmon ( Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis, Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8–2.0) routinely yielding 250–500 ng/µl (total 7.5–15 µg DNA from four to five tissue discs).