作者
João D Dias, Ilkka Liikanen, Kilian Guse, Johann Foloppe, Marta Sloniecka, Iulia Diaconu, Ville Rantanen, Minna Eriksson, Tanja Hakkarainen, Monika Lusky, Philippe Erbs, Sophie Escutenaire, Anna Kanerva, Sari Pesonen, Vincenzo Cerullo, Akseli Hemminki
发表日期
2010/5/1
期刊
Clinical Cancer Research
卷号
16
期号
9
页码范围
2540-2549
出版商
American Association for Cancer Research
简介
Purpose: Transfer of prodrug activation systems into tumors by using replication-deficient viruses has been suggested to be an effective method for achieving high local and low systemic anticancer drug concentrations. However, most current suicide gene therapy strategies are still hindered by poor efficiency of in vivo gene transfer, inefficient tumor penetration, limited bystander cell killing effect, and need of large prodrug doses. We hypothesized that local amplification provided by a replication competent platform would help overcome these limitations.
Experimental Design: We generated a transductionally and transcriptionally targeted oncolytic adenovirus Ad5/3-Δ24FCU1 expressing the fusion suicide gene FCU1. FCU1 encodes a bifunctional fusion protein that efficiently catalyzes the direct conversion of 5-FC, a relatively nontoxic antifungal agent, into the toxic metabolites 5-fluorouracil …
学术搜索中的文章
JD Dias, I Liikanen, K Guse, J Foloppe, M Sloniecka… - Clinical Cancer Research, 2010