作者
H Peter Reusch, Sven Zimmermann, Michael Schaefer, Martin Paul, Karin Moelling
发表日期
2001/9/7
期刊
Journal of Biological Chemistry
卷号
276
期号
36
页码范围
33630-33637
出版商
Elsevier
简介
The stimulation of platelet-derived growth factor (PDGF) receptors shifts vascular smooth muscle (VSM) cells toward a more proliferative phenotype. Thrombin activates the same signaling cascades in VSM cells, namely the Ras/Raf/MEK/ERK and the phosphatidylinositol 3-kinase (PI 3-kinase)/Akt pathways. Nonetheless, thrombin was not mitogenic, but rather increased the expression of the smooth muscle-specific myosin heavy chain (SM-MHC) indicative of anin vitro re-differentiation of VSM cells. A more detailed analysis of the temporal pattern and relative signal intensities revealed marked differences. The strong and biphasic phosphorylation of ERK1/2 in response to thrombin correlated with its ability to increase the activity of the SM-MHC promoter whereas Akt was only partially and transiently phosphorylated. By contrast, PDGF, a potent mitogen in VSM cells, induced a short-lived ERK1/2 phosphorylation but …
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HP Reusch, S Zimmermann, M Schaefer, M Paul… - Journal of Biological Chemistry, 2001