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Motivation: Mathematical morphology (MM) provides many powerful operators for processing 2D and 3D images. However, most MM plugins currently implemented for the popular ImageJ/Fiji platform are limited to the processing of 2D images.

Results: The MorphoLibJ library proposes a large collection of generic tools based on MM to process binary and grey-level 2D and 3D images, integrated into user-friendly plugins. We illustrate how MorphoLibJ can facilitate the exploitation of 3D images of plant tissues.

Availability and Implementation: MorphoLibJ is freely available at http://imagej.net/MorphoLibJ

Contact:  [email protected]

Supplementary information:  Supplementary data are available at Bioinformatics online.