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According to the latest economic theories, entrepreneurship has been understood as the fourth production factor. The activity degree of entrepreneurial subjects is differentiated by–among others–the character of the region they operate in. For a long time, the Olomouc region has been perceived as mostly an agricultural region with rather lower activity of entrepreneurial subjects. The aim of this paper is to map the activities of entrepreneurial subjects operating in the area of Olomouc region. 695 businesses from Olomouc region were monitored. As a research method, the desk research was used for data collection. Basic characteristics of entrepreneurial subjects in Olomouc region were found out via research and a follow-up analysis.