作者
Milka Koupenova, Heather A Corkrey, Olga Vitseva, Kahraman Tanriverdi, Mohan Somasundaran, Ping Liu, Shaukat Soofi, Rohan Bhandari, Matthew Godwin, Krishna Mohan Parsi, Alyssa Cousineau, René Maehr, Jennifer P Wang, Scott J Cameron, Jeffrey Rade, Robert W Finberg, Jane E Freedman
发表日期
2021/9/3
期刊
Circulation research
卷号
129
期号
6
页码范围
631-646
出版商
Lippincott Williams & Wilkins
简介
Rationale
Coronavirus disease 2019 (COVID-19) is characterized by increased incidence of microthrombosis with hyperactive platelets sporadically containing viral RNA. It is unclear if SARS-CoV-2 (severe acute respiratory syndrome, corona virus-2) directly alters platelet activation or if these changes are a reaction to infection-mediated global inflammatory alterations. Importantly, the direct effect of SARS-CoV-2 on platelets has yet to be studied.
Objective
To characterize the direct SARS-CoV-2–platelet interactions using in vitro studies with purified infectious virions and samples from infected patients.
Methods and Results
Platelet RNA analyzed by ARTIC v3 sequencing for SARS-CoV-2 showed presence of fragmented viral genome in all patients with COVID-19. Immunofluorescent imaging of platelets from patients with COVID-19 confirmed presence of SARS-CoV-2 proteins, whereas there was no detection …
学术搜索中的文章
M Koupenova, HA Corkrey, O Vitseva, K Tanriverdi… - Circulation research, 2021