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In several Wireless Sensor Networks applications, the sensitive nature of collected information makes the security of data transmission a major concern. Different cryptography techniques have been considered to provide reliable communication between nodes. However, the secure distribution of encryption keys is still a challenging task. In this work, we use physical layer key generation to simultaneously generate symmetric encryption keys at the end device and the gateway. Specifically, we present three contributions to the physical layer key generation process. Firstly, we propose a preprocessing method based on the difference between nonconsecutive Received Signal Strength Indicator (RSSI) samples. This preprocessing stage allows us to obtain random keys even in scenarios with low-mobility nodes. Secondly, we present a new quantization algorithm, named Lossy Cumulative Distribution Function (LCDF …