作者
Zhigang Xue, Kevin Huang, Chaochao Cai, Lingbo Cai, Chun-yan Jiang, Yun Feng, Zhenshan Liu, Qiao Zeng, Liming Cheng, Yi E Sun, Jia-yin Liu, Steve Horvath, Guoping Fan
发表日期
2013/8/29
期刊
Nature
卷号
500
期号
7464
页码范围
593-597
出版商
Nature Publishing Group UK
简介
Mammalian pre-implantation development is a complex process involving dramatic changes in the transcriptional architecture,,,. We report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos, using single-cell RNA sequencing. Based on single-nucleotide variants in human blastomere messenger RNAs and paternal-specific single-nucleotide polymorphisms, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25 to 53%). By weighted gene co-expression network analysis,, we find that each developmental stage can be delineated concisely by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation and metabolism, acting in a step-wise fashion from …
学术搜索中的文章
Z Xue, K Huang, C Cai, L Cai, C Jiang, Y Feng, Z Liu… - Nature, 2013