作者
Maria Alejandra Frias Martinez, Ísis de Fátima Balderrama, Paula Stephania Brandão Hage Karam, Rodrigo Cardoso de Oliveira, Flávia Amadeu de Oliveira, Carlos Roberto Grandini, Fábio Bossoi Vicente, Andreas Stavropoulos, Mariana Schutzer Ragghianti Zangrando, Adriana Campos Passanezi Sant’Ana
发表日期
2020/12
期刊
International journal of implant dentistry
卷号
6
页码范围
1-11
出版商
Springer Berlin Heidelberg
简介
Purpose
The aim of this study was to investigate the response of osteogenic cell lineage and gingival fibroblastic cells to different surface treatments of grade IV commercially pure Titanium (cpTi) disks.
Material and methods
Grade IV cpTi disks with different surfaces were produced: machined (M), sandblasting (B), sandblasting and acid subtraction (NP), and hydrophilic treatment (ACQ). Surface microtopography characteristics and chemical composition were investigated by scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS). Adhesion and proliferation of SC-EHAD (human surgically-created early healing alveolar defects) and HGF-1 (human gingival fibroblasts) on Ti disks were investigated at 24 and 48 h, and osteogenic differentiation and mineralization were evaluated by assessing alkaline phosphatase (ALP) activity and alizarin red staining, respectively.
Results
No …
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MAF Martinez, ÍF Balderrama, PSBH Karam… - International journal of implant dentistry, 2020