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Agent-based models (ABMs) are gaining traction as one of the most powerful modelling tools within the social sciences. They are particularly suited to simulating complex systems. Despite many methodological advances within ABM, one of the major drawbacks is their inability to incorporate real-time data to make accurate short-term predictions. This paper presents an approach that allows ABMs to be dynamically optimized. Through a combination of parameter calibration and data assimilation (DA), the accuracy of model-based predictions using ABM in real time is increased. We use the exemplar of a bus route system to explore these methods. The bus route ABMs developed in this research are examples of ABMs that can be dynamically optimized by a combination of parameter calibration and DA. The proposed model and framework is a novel and transferable approach that can be used in any passenger …