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Dual microchannel (MCh) assist photonic crystal fiber-based plasmonic sensor has been proposed to detect a maximum of two analytes simultaneously. The proposed sensor will improve the detection time and accuracy. The performance of the sensor is investigated using the finite element method. Due to the symmetric structure, the proposed sensor shows similar performance for both channels. The sensor can be applied for both cases single and multi-analyte detection. The sensor exhibits the maximum wavelength sensitivity (WS) of 11,000 nm/RIU and amplitude sensitivity (AS) of 922 RIU-1for y-polarized modes, respectively. Due to the multi-analyte detection ability, the proposed sensor will be a suitable candidate for real-time biological and organic chemicals detections.