作者
Taís S Assmann, Mariana Recamonde-Mendoza, Aline R Costa, Márcia Puñales, Balduíno Tschiedel, Luís H Canani, Andrea C Bauer, Daisy Crispim
发表日期
2019/1/28
期刊
Acta diabetologica
卷号
56
页码范围
55-65
出版商
Springer Milan
简介
Aims
The aim of this study was to investigate a miRNA expression profile in type 1 diabetes mellitus (T1DM) patients with DKD (cases) or without this complication (controls).
Methods
Expression of 48 miRNAs was screened in plasma of 58 T1DM patients (23 controls, 18 with moderate DKD, and 17 with severe DKD) using TaqMan Low Density Array cards (Thermo Fisher Scientific). Then, five of the dysregulated miRNAs were selected for validation in an independent sample of 10 T1DM controls and 19 patients with DKD (10 with moderate DKD and 9 with severe DKD), using RT-qPCR. Bioinformatic analyses were performed to explore the putative target genes and biological pathways regulated by the validated miRNAs.
Results
Among the 48 miRNAs investigated in the screening analysis, 9 miRNAs were differentially …
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TS Assmann, M Recamonde-Mendoza, AR Costa… - Acta diabetologica, 2019