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Intro: Transcriptome research has become increasingly popular to monitor global changes in gene expression. In particular, transcriptome differences between diseases versus healthy states can provide valuable insight into understanding immune response mechanisms. However, the isolation of high‐quality mRNA from tough tissues, such as skin, can represent a significant challenge in research. Herein, we compare two methods for mRNA extraction from two murine skin types with a specific focus on mRNA yields and integrity.

Methods: 1cm² full‐thickness mouse skin (epidermal and dermal layers) samples were freshly harvested, separated from fat, and kept on ice until homogenization. Samples were cut into fragments and placed in 1ml Trizol, then homogenized using a manual Dounce homogenizer on ice, or an automated Omni Bead Ruptor 24 (BR24) bead mill homogenizer without refrigeration. Clarified …