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In this paper, we propose and design a novel generator for the high voltage square pulses with the changeable polarities in order to reach the requirements and the needs for the intended experiments on sterilization of microorganism. The major component of our circuits is a non-trivial combination between a half bridge Marx generator and an H bridge in series, combining with the DSP control unit, which can adjust the voltages, frequencies, pulse widths and polarities, and also tolerate various loads. More precisely, we illustrate the design and operation principles for each module in the constructed circuit and analyze the system performance with various loads. The effectiveness and correctness of our proposed circuit and methodologies have been verified and demonstrated by both PSIM simulation results and our own prototype. In this paper, we also show the stability and reliability of the pulse generator we …