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Human induced pluripotent stem cells (iPSCs) have been shown as an attractive source for cartilage repair and regenerative medicine. Chondrogenesis of iPSCs is important in cartilage tissue engineering. The objective of our study was enhanced chondrogenic differentiation of iPSCs by using Poly-L-lactic acid/polyvinyl alcohol (PLLA/PVA)-based scaffold and chondrogenic medium. PLLA scaffold was fabricated via electrospinning. The iPSC cells were cultured on the PLLA/PVA scaffold and scaffold-free method. Real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. Also, we have done immunocytochemistry and SEM imaging for confirming differentiation. Real-time PCR showed that significantly increased gene expression of collagen type II, collagen type X and aggrecan on the PLLA/PVA scaffold method when compared to the mRNA levels measured in the scaffold-free …