作者
Tiong Kit Tan, Pramila Rijal, Rolle Rahikainen, Anthony H Keeble, Lisa Schimanski, Saira Hussain, Ruth Harvey, Jack WP Hayes, Jane C Edwards, Rebecca K McLean, Veronica Martini, Miriam Pedrera, Nazia Thakur, Carina Conceicao, Isabelle Dietrich, Holly Shelton, Anna Ludi, Ginette Wilsden, Clare Browning, Adrian K Zagrajek, Dagmara Bialy, Sushant Bhat, Phoebe Stevenson-Leggett, Philippa Hollinghurst, Matthew Tully, Katy Moffat, Chris Chiu, Ryan Waters, Ashley Gray, Mehreen Azhar, Valerie Mioulet, Joseph Newman, Amin S Asfor, Alison Burman, Sylvia Crossley, John A Hammond, Elma Tchilian, Bryan Charleston, Dalan Bailey, Tobias J Tuthill, Simon P Graham, Helen ME Duyvesteyn, Tomas Malinauskas, Jiandong Huo, Julia A Tree, Karen R Buttigieg, Raymond J Owens, Miles W Carroll, Rodney S Daniels, John W McCauley, David I Stuart, Kuan-Ying A Huang, Mark Howarth, Alain R Townsend
发表日期
2021/1/22
期刊
Nature communications
卷号
12
期号
1
页码范围
542
出版商
Nature Publishing Group UK
简介
There is need for effective and affordable vaccines against SARS-CoV-2 to tackle the ongoing pandemic. In this study, we describe a protein nanoparticle vaccine against SARS-CoV-2. The vaccine is based on the display of coronavirus spike glycoprotein receptor-binding domain (RBD) on a synthetic virus-like particle (VLP) platform, SpyCatcher003-mi3, using SpyTag/SpyCatcher technology. Low doses of RBD-SpyVLP in a prime-boost regimen induce a strong neutralising antibody response in mice and pigs that is superior to convalescent human sera. We evaluate antibody quality using ACE2 blocking and neutralisation of cell infection by pseudovirus or wild-type SARS-CoV-2. Using competition assays with a monoclonal antibody panel, we show that RBD-SpyVLP induces a polyclonal antibody response that recognises key epitopes on the RBD, reducing the likelihood of selecting neutralisation-escape …
学术搜索中的文章
TK Tan, P Rijal, R Rahikainen, AH Keeble… - Nature communications, 2021