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The aim of the present study was to assess Amorphophallus paeoniifolius tuber and its peel for its antibacterial activity. The tuber and peel extracts of the selected plant were tested against five pathogenic bacteria. The ethanolic tuber extract of the plant shown inhibitory effect on four bacterial species such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Streptococcus mutans. The ethanolic tuber extract displayed no effect on Bacillus subtilis. The ethanolic extract of tuber showed diameter of inhibition zones ranging from 6 mm-18 mm. The ethanolic extract of peel showed inhibitory effect only on two bacterial species such as Staphylococcus aureus and Pseudomonas aeruginosa. The ethanolic extract of peel exhibited inhibition zone ranging from 7 mm to 16 mm. Water extracts of tuber (inhibition zone ranging from 7 mm to 9mm) and peel (inhibition zone ranging from 6 mm to 9 mm) inhibited only one bacterial species such as Staphylococcus aureus and Streptococcus mutans respectively.