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Cordycepin is an adenosine analogue isolated from the fungus Cordyceps militaris. Cordycepin is a nucleoside antimetabolite that has shown a broad spectrum of biological activity including antineoplastic activity. limited research has been carried out on the effects of Cordycepin on the regenerative potential of stem cells, including dental pulp-derived mesenchymal stem cells. The present study was designed to assess if Cordycepin could enhance the vital properties of dental pulp-derived mesenchymal stem cells for regenerative purposes.

Objective: To examine the effect of Cordycepin on the viability, proliferation, and migratory properties of dental pulp-derived mesenchymal stem cells. Materials and methods: The pulp was derived from human premolar teeth extracted for orthodontic purposes after obtaining informed consent. The samples were transferred to the laboratory for processing. DPSCs were expanded and characterized using flow cytometry and differentiation to the bone, adipose, and cartilage cells was examined. MTT Assay was performed using various concentrations of Cordycepin. The growth curve was plotted for 13 days. Cell cycle analysis was performed by flow cytometry. Migratory ability was assessed by wound healing assay. ROS generation was detected by flow cytometry. Gene expression was quantified by RT-qPCR. Statistical analysis was performed. p < 0.05 was considered as significant and p < 0.01 was considered as highly significant (* p < 0.05, and ** p < 0.01). Results: DPSCs expressed characteristic MSC-specific markers and trilineage differentiation …