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This study examined the impact of personality traits on the knowledge sharing behavior of academicians in the public sector. The data were collected from 237 respondents using a questionnaire. The results showed that the personality trait openness to experience had a significant and positive impact on the knowledge sharing behavior and its sub-dimensions such as written contributions, organizational communication, personal interactions, and communities of practice. Furthermore, the personality traits extraversion and agreeableness positively predicted the knowledge sharing behavior for the dimensions of communities of practice and organizational communication, respectively. The results of this study would be helpful for the administrative staff of universities to develop programs to promote a knowledge sharing culture in universities and improve collaborative learning, research, organizational effectiveness …