作者
Dana J Holger, Katherine L Lev, Razieh Kebriaei, Taylor Morrisette, Rahi Shah, Jose Alexander, Susan M Lehman, Michael J Rybak
发表日期
2022/9/1
期刊
Journal of Applied Microbiology
卷号
133
期号
3
页码范围
1636-1649
出版商
Blackwell Science Ltd
简介
Aims
Here, we investigate the impact of phage‐antibiotic combinations (PAC) on bacterial killing, resistance development and outer membrane vesicle (OMV) production in multidrug‐resistant (MDR) P. aeruginosa.
Methods and Results
After screening 10 well‐characterized MDR P. aeruginosa strains against three P. aeruginosa phages, representative strains, R10266 and R9316, were selected for synergy testing based on high phage sensitivity and substantial antibiotic resistance patterns, while phage EM was chosen based on host range. To understand the impact of phage‐antibiotic combinations (PAC) against MDR P. aeruginosa, time‐kill analyses, OMV quantification and phage/antibiotic resistance testing were performed. Phage and meropenem demonstrated synergistic activity against both MDR strains. Triple combination regimens, phage‐meropenem‐colistin and …
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DJ Holger, KL Lev, R Kebriaei, T Morrisette, R Shah… - Journal of Applied Microbiology, 2022