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To improve transportation safety, this study applies Highway Safety Manual (HSM) procedures to roadways while accounting for unobserved heterogeneity and exploring alternative functional forms for Safety Performance Functions (SPFs). Specifically, several functional forms are considered in Poisson and Poisson-gamma modeling frameworks. Using 5 years (2011–2015) of crash, traffic, and road inventory data for two-way, two-lane roads in Tennessee, fixed- and random-parameter count data models are calibrated. The models account for important methodological concerns of unobserved heterogeneity and omitted variable bias. With a validation dataset, the calibrated and uncalibrated HSM SPFs and eight new Tennessee-specific SPFs are compared for prediction accuracy. The results show that the statewide calibration factor is 2.48, suggesting rural two-lane, two-way road segment crashes are at least 1.48 …