作者
Mitchell J Sullivan, Deena R Altman, Kieran I Chacko, Brianne Ciferri, Elizabeth Webster, Theodore R Pak, Gintaras Deikus, Martha Lewis-Sandari, Zenab Khan, Colleen Beckford, Angela Rendo, Flora Samaroo, Robert Sebra, Ramona Karam-Howlin, Tanis Dingle, Camille Hamula, Ali Bashir, Eric Schadt, Gopi Patel, Frances Wallach, Andrew Kasarskis, Kathleen Gibbs, Harm van Bakel
发表日期
2019/12
期刊
Journal of clinical microbiology
卷号
57
期号
12
页码范围
10.1128/jcm. 01261-19
出版商
American Society for Microbiology
简介
Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part of infection prevention practices. In this study, we established a long-read technology-based WGS screening program of all first-episode methicillin-resistant Staphylococcus aureus (MRSA) blood infections at a major urban hospital. A survey of 132 MRSA genomes assembled from long reads enabled detailed characterization of an outbreak lasting several months of a CC5/ST105/USA100 clone among 18 infants in a neonatal intensive care unit (NICU). Available hospital-wide genome surveillance data traced the origins of the outbreak to three patients admitted to adult wards during a 4-month period preceding the NICU outbreak. The pattern of changes among complete outbreak genomes provided full spatiotemporal resolution of its progression, which was characterized by multiple subtransmissions and likely precipitated …
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MJ Sullivan, DR Altman, KI Chacko, B Ciferri… - Journal of clinical microbiology, 2019