作者
João GS Souza, Barbara E Costa Oliveira, Martinna Bertolini, Carolina Veloso Lima, Belén Retamal‐Valdes, Marcelo de Faveri, Magda Feres, Valentim AR Barão
发表日期
2020/4
期刊
Journal of periodontal research
卷号
55
期号
2
页码范围
258-266
简介
Objective
To evaluate the effect of titanium (Ti) particles and ions on oral biofilm growth and composition.
Background
Particles and ions of Ti released from dental implants can trigger unfavorable biological responses in human cells. However, their effect on oral biofilms composition has not been tested.
Methods
In this blind in situ study, volunteers wore a palatal appliance containing Ti disks for 7 days to allow biofilm formation. Disks were then collected and biofilms were treated, in vitro, with Ti particles (0.75% and 1%), ions (10 and 20 ppm), or a combination of both (1% particles + 20 ppm ions). Biofilms exposed only to medium was used as control group. After 24 hours, biofilms were collected and analyzed by checkerboard DNA‐DNA hybridization. Direct effects of Ti particles and ions on biofilm/cellular morphology were evaluated by transmission electron microscopy (TEM).
Results
Ti particles affected …
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JGS Souza, BE Costa Oliveira, M Bertolini, CV Lima… - Journal of periodontal research, 2020