作者
Daniel JM Fernández-Ayala, Sergio F Martín, María P Barroso, Consuelo Gómez-Díaz, José M Villalba, Juan C Rodríguez-Aguilera, Guillermo López-Lluch, Plácido Navas
发表日期
2000/6
期刊
Antioxidants & redox signaling
卷号
2
期号
2
页码范围
263-275
出版商
Mary Ann Liebert, Inc.
简介
Coenzyme Q10 (CoQ10) is a component of the antioxidant machinery that protects cell membranes from oxidative damage and decreases apoptosis in leukemic cells cultured in serum-depleted media. Serum deprivation induced apoptosis in CEM-C7H2 (CEM) and to a lesser extent in CEM-9F3, a subline overexpressing Bcl-2. Addition of CoQ10 to serum-free media decreased apoptosis in both cell lines. Serum withdrawal induced an early increase of neutral-sphingomyelinase activity, release of ceramide, and activation of caspase-3 in both cell lines, but this effect was more pronounced in CEM cells. CoQ10 prevented activation of this cascade of events. Lipids extracted from serum-depleted cultures activated caspase-3 independently of the presence of mitochondria in cell-free in vitro assays. Activation of caspase-3 by lipid extracts or ceramide was prevented by okadaic acid, indicating the implication of a …
引用总数
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DJM Fernández-Ayala, SF Martín, MP Barroso… - Antioxidants & redox signaling, 2000