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A study has been conducted on Acoustic Parameters of Spindle oil-Ethanol system, on different concentration’s [0.2%, 0.4%, 0.6%, 0.8% and 1.6%] at temperature of 303K using Ultrasonic interferometer. Parameters like Ultrasonic velocity, Viscosity, Density, Adiabatic compressibility, Acoustic Impedance, Free length, Free Volume, Relation Time, Relative Association, Adsorption Coefficient and Molar compressibility are measured and it is helpful for correlation of Molecular interaction of binary mixture spindle oil-ethanol system.