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Research and development of heterogeneous small cell in cellular network accommodates the proliferation of data-hungry devices and applications. Meanwhile, for challenges in providing high-data-rate transmission in poor coverage area, utilizing unmanned aerial vehicles (UAVs) provides a promising solution attracting tremendous attention. However, tight integration of UAVs creates an obstruction in existing network to acquire high efficient resource utilization, which needs investigation in 3 D network architecture. In this letter, we propose a resource allocation optimization mechanism to minimize mean packet transmission delay in 3 D cellular network with multi-layer UAVs. Numerical results demonstrate effectiveness of the proposed algorithm, where optimal spectrum and power allocation can provide minimum packet transmission delay.