作者
Atsuhiko Kawamoto, Tengis Tkebuchava, Jun-Ichi Yamaguchi, Hiromi Nishimura, Young-Sup Yoon, Charles Milliken, Shigeki Uchida, Osamu Masuo, Hideki Iwaguro, Hong Ma, Allison Hanley, Marcy Silver, Marianne Kearney, Douglas W Losordo, Jeffrey M Isner, Takayuki Asahara
发表日期
2003/1/28
期刊
Circulation
卷号
107
期号
3
页码范围
461-468
出版商
Lippincott Williams & Wilkins
简介
Background— We investigated whether catheter-based, intramyocardial transplantation of autologous endothelial progenitor cells can enhance neovascularization in myocardial ischemia.
Methods and Results— Myocardial ischemia was induced by placement of an ameroid constrictor around swine left circumflex artery. Four weeks after constrictor placement, CD31+ mononuclear cells (MNCs) were freshly isolated from the peripheral blood of each animal. After overnight incubation of CD31+ MNCs in noncoated plates, nonadhesive cells (NA/CD31+ MNCs) were harvested as the endothelial progenitor cell–enriched fraction. Nonadhesive CD31− cells (NA/CD31− MNCs) were also prepared. Autologous transplantation of 107 NA/CD31+ MNCs, 107 NA/CD31− MNCs, or PBS was performed with a NOGA mapping injection catheter to target ischemic myocardium. In a parallel study, 105 human CD34+ MNCs, 10 …
引用总数
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A Kawamoto, T Tkebuchava, JI Yamaguchi… - Circulation, 2003