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Urbanization is linked to economic growth, and agglomeration economies mean that people in larger cities are more productive. However, urban expansion is also associated with congestion, localized environmental damage, and potentially, social segregation. In this paper we examine how urban expansion and changing urban spatial structure affects the level and scale of socioeconomic segregation of cities in Mexico. We measure different dimensions of urban spatial structure, and segregation by income and education at different geographic scales in 100 Mexican cities from 1990 to 2010. We then examine correlations between the two sets of variables, and run multivariate regressions to assess how changes in urban spatial structure relate to changes in the level and scale of segregation. Findings reveal that as cities expand, inhabitants experience greater levels of socio-economic segregation, especially at a …