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This study evaluated the effect of supplementing different levels of wheat bran with sugarcane bagasse on the production of pink oyster mushroom (Pleurotus djamor) and find out their yield and proximate composition. The sugarcane bagasse was mixed at spawning with 0%, 10%, 20%, 30%, 40%, and 50% of wheat bran supplement and arranged in a complete randomized design with three replications, three spawn packets in each replication and six treatments. Result showed significantly (P< 0.01) different, the highest mycelium running rate was observed on 40% and lowest on 0% due to using different levels of wheat bran supplement. Number of days from stimulation to primordia initiation and harvest, sugarcane bagasse supplemented with 30% wheat bran took longer time (5.5 days) for primordia and (5 days) to harvest, while 20% took shorter time (6.8 days) to full colonization. 10% supplement provided highest result in term of average number of primordia/packet (176.3), fruiting body/packet (77.6) and weight/fruiting body (5.3 g). The growth of pileus and stipe were significantly (P< 0.05 (stipe length, pileus thickness) and P< 0.01 (diameter)) different, being highest diameter on 30%, stripe length on 10% and pileus thickness on 40%. The yields of pink oyster mushroom showed significantly (P< 0.01) different result, biological yield, economic yield, dry yield, biological efficiency and cost benefit ratio were obtained higher with 10% wheat bran supplement. The maximum moisture content was determined on 50%, dry matter, ash on 20% and protein content, crude fiber on 30%. 50% supplement was accounted for the highest amount of …