作者
Colin D Funk, Lena B Funk, Matthew E Kennedy, Amy S Pong, Garret A Fitzgerald
发表日期
1991/6
期刊
The FASEB journal
卷号
5
期号
9
页码范围
2304-2312
简介
Platelets metabolize arachidonic acid to thromboxane A2, a potent platelet aggregator and vasoconstrictor compound. The first step of this transformation is catalyzed by prostaglandin (PG) G/H synthase, a target site for nonsteroidal antiinflammatory drugs. We have isolated the cDNA for both human platelet and human erythroleukemia cell PGG/H synthase using the polymerase chain reaction and conventional screening procedures. The cDNA encoding the full‐length protein was expressed in COS‐M6 cells. Microsomal fractions from transfected cells produced prostaglandin endoperoxide‐derived products which were inhibited by indomethacin and aspirin. Mutagenesis of the serine residue at position 529, the putative aspirin acetylation site, to an asparagine reduced cyclooxygenase activity to barely detectable levels, an effect observed previously with the expressed sheep vesicular gland enzyme. Platelet …
引用总数
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CD Funk, LB Funk, ME Kennedy, AS Pong… - The FASEB journal, 1991