作者
Stephan J Ott, Nour Eddine El Mokhtari, Meike Musfeldt, Stephan Hellmig, Sandra Freitag, Ateequr Rehman, Tanja Kühbacher, S Nikolaus, Pawel Namsolleck, Michael Blaut, Jochen Hampe, Hany Sahly, Alexander Reinecke, Nils Haake, Rainer Günther, Dietmar Krüger, Markus Lins, Gunhild Herrmann, Ulrich R Fölsch, Rüdiger Simon, Stefan Schreiber
发表日期
2006/2/21
期刊
Circulation
卷号
113
期号
7
页码范围
929-937
出版商
Lippincott Williams & Wilkins
简介
Background— Bacterial infection has been discussed as a potential etiologic factor in the pathophysiology of coronary heart disease (CHD). This study analyzes molecular phylogenies to systematically explore the presence, frequency, and diversity of bacteria in atherosclerotic lesions in patients with CHD.
Methods and Results— We investigated 16S rDNA signatures in atherosclerotic tissue obtained through catheter-based atherectomy of 38 patients with CHD, control material from postmortem patients (n=15), and heart-beating organ donors (n=11) using clone libraries, denaturating gradient gel analysis, and fluorescence in situ hybridization. Bacterial DNA was found in all CHD patients by conserved PCR but not in control material or in any of the normal/unaffected coronary arteries. Presence of bacteria in atherosclerotic lesions was confirmed by fluorescence in situ hybridization. A high overall bacterial …
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SJ Ott, NE El Mokhtari, M Musfeldt, S Hellmig, S Freitag… - Circulation, 2006