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Scholars argue that bridging students’ backgrounds with canonical science is necessary for students of color by reducing incongruences between home and school and increasing the authenticity of science learning. However, science teachers often struggle with enacting culturally responsive pedagogies (CRP). This paper utilizes data from classroom observations, group interviews, and numerous program artifacts to describe four themes associated with the knowledge and practices of six high school life science teachers as they learned about and attempted to enact CRP while participating in a professional development program. These themes include views of students that changed from a focus on indirect experiences and emphasizing what students lacked to direct experiences and discussions of resources that they brought to the classroom; repositioning of students and teachers that was originally …