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ZnO nanoparticles are well known multifunctional inorganic nanoparticles with predominant antimicrobial potential. In the current study, ZnO nanoparticles were synthesized and characterized by UV–Vis and Fourier transform infrared (FTIR) spectroscopy, shape and size of the nanoparticles were analyzed with the help of electron microscope. The nanoparticles were evaluated for antimicrobial potential against Salmonella typhimurium and Staphylococcus aureus by kill time analysis. Further quantification of bacterial response was evaluated by measuring minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC). UV–Vis and FTIR spectra conformed the formation of ZnO nanoparticles, whereas, the transmission electron microscope (TEM) showed that the average size of the synthesized nanoparticles was in the range of 20 nm. ZnO nanoparticles showed significant (p < 0.05 …