作者
Li-Wei Shi, Zhi-Bo Zhao, Li Zhong, Jian Gao, Jian-Ping Gong, Hang Chen, Yu Min, Yi-Yin Zhang, Zhi Li
发表日期
2020/10/14
期刊
Cancer Management and Research
页码范围
10139-10150
出版商
Taylor & Francis
简介
Background
Rab40b is an evolutionarily conserved Rab GTPase that plays an important role in intracellular trafficking and is closely related to cancer progression. However, the role and potential molecular mechanism of Rab40b in hepatocellular carcinoma (HCC) have not yet been elucidated.
Materials and Methods
The expression of Rab40b in HCC tissues and peritumour tissues was tested by qRT-PCR, Western blotting and histological analysis. A Kaplan–Meier survival curve was generated based on the expression of Rab40b in the HCC samples. Cell proliferation assays, wound healing assays, and transwell assays are used to examine the effect of Rab40b on HCC cell growth in vitro. The effect of Rab40b on cell cycle was examined by flow cytometry. A xenograft implantation model was used to assess the effect of Rab40b on the development of HCC cells in vivo.
Results
Rab40b protein expression is …
学术搜索中的文章
LW Shi, ZB Zhao, L Zhong, J Gao, JP Gong, H Chen… - Cancer Management and Research, 2020