作者
Kiyoshi Mashiguchi, Keita Tanaka, Tatsuya Sakai, Satoko Sugawara, Hiroshi Kawaide, Masahiro Natsume, Atsushi Hanada, Takashi Yaeno, Ken Shirasu, Hong Yao, Paula McSteen, Yunde Zhao, Ken-ichiro Hayashi, Yuji Kamiya, Hiroyuki Kasahara
发表日期
2011/11/8
期刊
Proceedings of the National Academy of Sciences
卷号
108
期号
45
页码范围
18512-18517
出版商
National Academy of Sciences
简介
The phytohormone auxin plays critical roles in the regulation of plant growth and development. Indole-3-acetic acid (IAA) has been recognized as the major auxin for more than 70 y. Although several pathways have been proposed, how auxin is synthesized in plants is still unclear. Previous genetic and enzymatic studies demonstrated that both TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) and YUCCA (YUC) flavin monooxygenase-like proteins are required for biosynthesis of IAA during plant development, but these enzymes were placed in two independent pathways. In this article, we demonstrate that the TAA family produces indole-3-pyruvic acid (IPA) and the YUC family functions in the conversion of IPA to IAA in Arabidopsis (Arabidopsis thaliana) by a quantification method of IPA using liquid chromatography–electrospray ionization–tandem MS. We further show that YUC protein expressed …
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K Mashiguchi, K Tanaka, T Sakai, S Sugawara… - Proceedings of the National Academy of Sciences, 2011