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In this paper, the removal of two anionic dyes from textile effluent in single and binary systems was investigated. Direct Red 23 and Acid Green 25 were used as anionic dyes. The surface characteristics of chitosan were investigated using Fourier transform infrared. The effects of operational parameters such as chitosan dosage, initial dye concentration, salt and pH on dye removal were studied. The isotherms of dye adsorption were investigated. It was found that the isotherm data of Direct Red 23 and Acid Green 25 in single and binary systems followed Tempkin isotherm. In addition adsorption kinetics of dyes was studied in single and binary systems and rate sorption was found to conform to pseudo-second order kinetics with a good correlation. Results indicated that chitosan could be used as a biosorbent to remove the anionic dyes from contaminated watercourses in both single and binary systems of pollutants.