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Recently, nanotechnology as novel interdisciplinary sciences has been introduced among all fields and gets numerous attentions, due to its unique applications. In biomedical engineering, electrospinning exhibits a lot of advantages as a nanofibers scaffolds producer, which can make appropriate resemblance in physical structure with extra cellular matrix (ECM). This is because of the nanometer scale of ECM fibrils in diameter, which can be mimicked by electrospinning procedure as well as its porous structure. In this review, we attempt to explore the electrospun nanofibers scaffolds applications in biomedical engineering.