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Background: Given the wide application of autologous fat grafting, a new emphasis on fat processing techniques has emerged in an effort to limit unpredictable degrees of resorption often seen with this procedure. With the growing interest in regenerative medicine, approaches to supplement fat grafts with adipose-derived stem cells are evolving in hopes of promoting vascularization and neoadipogenesis.

Objective: The authors evaluated the outcomes of the most common processing techniques for fat grafting—decantation, washing, high-speed centrifugation—and stromal vascular cell-supplemented lipotransfer to determine which method yields a higher percentage of retention and better quality graft.

Methods: A total of 32 subcutaneous injections of processed human lipoaspirate were carried out in 8 athymic rats. Each animal received all 4 processing conditions, with end points at 4, 8 …