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Shear-based material removal processes significantly influence the quality of workpiece surface and implicitly the component functional performance. An in-situ SEM nano-cutting enabled the study of crystal flow and lattice rotation occurring below the cutting edge in a polycrystalline Nickel superalloy. When nano-cutting within single grains a deformed nanolayer appears that consists of a crystal lattice rotated exclusively within the cutting plane which is delimited from the bulk of the grain by high angle boundary (HAB); the depth of deformed nanolayer increases with the material pile-up (nano-chip) caused by the grain shearing. Upon nano-cutting multiple grains, nano-recrystallisation at the HAB occurs, accompanied by the bending of the grain boundary (GB) in the cutting direction, a phenomenon that also significantly influences the deformation behaviour of the grains cut after passing the GB. Clarifying these …