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We reported ordered porous poly(l-lactic acid) (PLLA) piezoelectric nanofibers on comb electrode for both a stain sensing and human joint motion energy harvesting. The ordered porous PLLA piezoelectric nanofibers were fabricated using electrospinning equipment. The reason for choosing this equipment was that the electric dipole component along the main carbon chain of PLLA polymer nanofibers can be polarized along the direction of alignment during the process of a direct current electric field electrospinning. When the ordered porous PLLA piezoelectric nanofibers prototype is motioned with a bending deformation, the produced charges on PLLA piezoelectric nanofibers will flow out through comb electrode for a measurement. It demonstrated that an open-circuit voltage and short-circuit current with a strain deformation angle 28.9° could reach 0.55 V and 230 pA, respectively. It concluded that the …