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Physical techniques for the detection of singlet oxygen are based on monomol or dimol emission. This chapter reports on applications using an approach to determine the singlet oxygen (¹O₂) quenching constant by measuring steady-state monomol photoemission of singlet oxygen at 1270 nm detected by a germanium diode photodetector. Overall singlet oxygen quenching constants—the sum of the rate constants for chemical reaction and physical quenching—can be calculated from the decrease in singlet oxygen photoemission. Time-resolved luminescence spectroscopy allows determination of the effect of tested compounds on the lifetime of singlet oxygen generated photochemically by pulse irradiation of a photosensitizer. Singlet oxygen formed by photo-excitation in skin on ultraviolet A exposure or in dark reactions by stimulated phagocytes has damaging effects on biomolecules and …