作者
Alessia Fornoni, Jongmin Jeon, Javier Varona Santos, Lorenzo Cobianchi, Alexandra Jauregui, Luca Inverardi, Slavena A Mandic, Christina Bark, Kevin Johnson, George McNamara, Antonello Pileggi, R Damaris Molano, Jochen Reiser, Karl Tryggvason, Dontscho Kerjaschki, Per-Olof Berggren, Peter Mundel, Camillo Ricordi
发表日期
2010/1/1
期刊
Diabetes
卷号
59
期号
1
页码范围
190-199
出版商
American Diabetes Association
简介
OBJECTIVE
Nephrin, an immunoglobulin-like protein essential for the function of the glomerular podocyte and regulated in diabetic nephropathy, is also expressed in pancreatic β-cells, where its function remains unknown. The aim of this study was to investigate whether diabetes modulates nephrin expression in human pancreatic islets and to explore the role of nephrin in β-cell function.
RESEARCH DESIGN AND METHODS
Nephrin expression in human pancreas and in MIN6 insulinoma cells was studied by Western blot, PCR, confocal microscopy, subcellular fractionation, and immunogold labeling. Islets from diabetic (n = 5) and nondiabetic (n = 7) patients were compared. Stable transfection and siRNA knockdown in MIN-6 cells/human islets were used to study nephrin function in vitro and in vivo after transplantation in diabetic immunodeficient mice. Live imaging of green …
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A Fornoni, J Jeon, J Varona Santos, L Cobianchi… - Diabetes, 2010