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In this paper we discuss the potential effects of using various segment durations of the available contents within a simulated mobile environment. OPNET modeler was used to implement a real-time test platform for mobile video streaming using OPNET system in the loop component (SITL). The real-time test platform was then used to evaluate the performance of the requests for different segment size. Dynamic Adaptive Streaming over HTTP (DASH) protocol was used to provide different test cases for video content with different segment sizes. In our experiment, we used different segments of different sizes (4, 8, and 12 sec.). Our comparison criteria include client download throughput (Bandwidth utilization), and CPU consumption. We identify the different trade-offs to be made in order to optimize the usage of available resources.