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The environment project in the greater Mekong sub-region was the largest multi-field environmental cooperation launched by six countries (China, Vietnam, Laos, Myanmar, Thailand and Cambodia) in 2006, since the cooperation mechanism was established by Asian Development Bank (ADB) in 1992. How to establish the indicators to assess the achievements of the biological corridor construction and the status of ecological environment quantitatively is one of the prerequisites for the future project ongoing phase. The popular Pressure-State-Response (PSR) framework was employed in this study to assess the natural and human pressure, the healthy state of regional natural environment, and the subsequent response of ecosystem dynamic change in the Greater Mekong Subregion. Instead of using surveying based data as driving parameters, large amount of driving factors were retrieved from multi-source …